Saxenian’s Argument Essay Example for Free

Saxenian’s Argument Essay Saxenian conducted a comparative study of two regions that focused on electronic industry; Silicon Valley and Route 128. Between these two, reports showed that as of 1990, Silicon Valley emerged as more competitive and successful than Route 128. Saxenian conducted interviews in the area and found out that the employee mobility in Silicon Valley was higher. To argue that such mobility is the reason why Silicon Valley succeeds was something that defies the common knowledge and principles of economics. Such action would inevitably lead to a leakage of information across different organizations and might affect competition. When one company get holds of the other company’s innovative knowledge, they would apply the knowledge to their own company or use it towards their own advantage. Another observation that was made by Saxenian was the prevalence of collaboration in their network-based system and the promotion of â€Å"open-markets that encourage competition and entrepreneurship†. Through network-based system, knowledge was spread horizontally. The presence of new organizations or corporations are welcomed and supported. On the other hand, in Route 128 wherein companies are integrated to the point that competition becomes limited to the largest firms. Knowledge and authority â€Å"flows vertically† or stay within the firm. Nonetheless, through the development of the internet and other telecommunication technology, Saxonian’s argument no longer holds for a similar comparison today. The internet permits employees and employers among different firms or agencies to communicate. Cluster networks in a particular regional economy can communicate or exchange information with firms or companies abroad. Knowledge is retrieved more promptly and more accurately today through internet search engines. Job hopping or employee mobility was not a major factor today than before since information are more readily available than 20 years ago.

Explain the new Features of the New Deal Essay Example for Free

Explain the new Features of the New Deal Essay In October 1929 the Wall Street stock market crashed in New York and panic selling of shares caused a wave of bankruptcies, destroying all confidence in businesses and investors. The great Depression led to 13 million people being unemployed. President Hoover, the government did nothing at all to help the Americans, he didn’t even think of helping them rebuild the American economy. In 1932 president Roosevelt came into power in the presidential elections. He had many concerns and considerations for the Americans and the economy. The first stage of the act of the New Deal to put the US back to work and create jobs in 1933 he introduced the first Acts during the hundred days, which involved the creation of Alphabet Agencies to deal with the economic problems. By 1935, the Supreme Court thought some of the New Deal laws as interfering too much. , so then Roosevelts response was to introduce the second stage of reform- The second New Deal. The 3 aims of the New Deal were: Relief; Recovery and Reform. Which were introduced by Roosevelt- the three ‘R’s’. Relief was measures to help relieve the suffering of the unemployed. Recovery was to try to rebuild the depression-shattered economy and Reform was aimed to give workers better working conditions and fairer wages. The aim of the relief was achieved by the: Civilian Conservation Corps (CCC); Federal Emergency Relief Administration (FERA); public works Administration (PWA); Home owners Loans Corporation (HOLC) and the works Progress Administration (WPA). The Civilian Conservation Act was set up to provide work for unemployed men under 25 years by encouraging them to work on projects in the countryside such as fish farming, planting trees and strengthening river banks. They also organised the army. The PWA stood for Public Works Administration, their aim was to use Unemployed unskilled workers to work on a large scale of public construction such as roads and bridges. The main part of the act was to set up public construction for the large scale building work, to build schools, hospitals and city halls. The FERA was set up to stop the threat of starvation that existed by making grants to local and state governments to help them give relief to the unemployed. Roosevelt thought that the right way to help the unemployed was to put them back to work after the threat of starvation was over instead of just giving them money. The HOLC stood for Home Owners Loans Corporation, the aim of this act was to ensure that people could continue to repay their mortgages. The government gave low interest rates to home owners to allow them to continue paying their mortgages until they were unemployed. The WPA stood for Works Progress Administration. Their aim was to find emergency short-term employment for unskilled workers in construction projects. The Acts that were part of the New Deal and helped with Recovery were: The Emergency Banking Act; the Securities Act; the Agricultural Adjustment Administration (AAA); the National Recovery Administration (NRA) and the Tennessee Valley Authority (TVA). The Emergency Banking Act aimed to solve the financial crisis after the Wall Street Crash by forcing all banks to remain close for 4 days. This actually worked and saved all the banks. The Securities Act was about providing full information about the companies issuing new shares, to the public. This established with a Securities and Exchange Commission in 1934 which was giving powers to control the activities of the stock market. It was the AAA’s duty to solve agriculture’s most serious problem which was over-production. Prices were low and farmers were unable to make a decent living. Roosevelt paid farmers to stop producing. The AAA gave the government power to destroy surplus food and give farmers compensation. The NRA stood for National Recovery Administration; they tried to create partnership between government and Industry to get rid of Child Labour, Long hours and Low pay. Each industry would agree an employment code with a government. The code was to guarantee workers fairer working conditions and wages. The TVA stood for Tennessee Valley Authority. The main parts of the act was to help encourage industry come to the hard hit valley and to build damn to use hydroelectric power of the river. Finally, there were parts of the New Deal that helped to reform the USA. They were: Collecting Bargaining; the Wagner Act and the social securities Act. Collective bargaining was where workers had the right to collective bargaining of wages. This gave an enormous boost to the trade unions. The Wagner act (1935) was the given right for workers to join the trade union which restored protection for the workers also. The Social Securities Act was introduced in 1935. This provided old age pensions, unemployment benefits and financial support for the handicapped. It was funded by contributions paid by workers, employers and the government. In Conclusion, when Roosevelt came into power he aimed to achieve relief, recovery and reform. The CCC, FERA, WPA and the HOLC. The emergency banking act, the Securities Act, NRA, AAA and the WPA achieved recovery. Collective Bargaining, Wagner Act and the Social Securities Act achieved reform. These Acts were all produced to help America by these 3 aims.

An Interesting Definition Of Mature Product Marketing Essay

An Interesting Definition Of Mature Product Marketing Essay An interesting definition of mature product market is found in Economics in Business Context by Colin Haslam, Alan Neale and Sukhdev Johal (2002, p.92). As per this definition, a product market reaches maturity when demand for the product is determined by replacement. I will try to elaborate this definition. In a mature product market, there is little or no scope for the further growth of the product. In simple words, the product has been sold to the maximum number of targeted customers. Hence, customers will demand the product only when the previously bought product has to be replaced. Does maturity necessarily lead to deterioration of market? This question can be answered using the concept of cyclicality. Economics in Business Context (Haslam et al. 2002, p.92) further defines cyclical markets as generally mature markets in which volume fluctuates at or around steady pattern of demand. Depending on the replacement period of the product, the product will be required by the customers every few years, months or weeks. During the period when maximum customers replace the product, the demand for the product will be at its positive peak. This period is followed by a period of low sales. Thus, fluctuation in the demand for a mature product makes it cyclical. Some products have longer replacement periods than the others, making them cyclical. For example, a refrigerator may be replaced after every ten years but cold drinks manufactured by Coco Cola or Pepsi are bought by billions of people every day. Therefore, refrigerators may be termed as mature and cyclical. Coco Cola and Pepsi have reached maturity but they are not cyclical. Let me illustrate with the help of a hypothetical example. Consider a product XYZ. Say, I had introduced XYZ in the market in 1990. The replacement period of this product was five years. Using excellent marketing strategy, XYZ had gained popularity amongst the customers by 1991. But, I had not yet sold XYZ to maximum number of targeted customers. I had to maximize my sales as well as confront a new problem; a competitor introduced a similar product called ABC in the market in the year 1992. After a research on XYZ, I realized that the product could be manufactured in a better way, leading to an increase its durability. The replacement period after increase in durability became 8 years. Increase in the durability of XYZ attracted more number of customers. Because it was a very popular product, XYZ reached maturity by 1995. It was sold to maximum number of customers. If I had not increased the durability of XYZ, more number of people would have bought XYZ every few years. Thus, the product was sold to maximum number of customers in its initial years in the market. But later, maturity, increase in durability and competition in the market led to a drop in the sales. After this, the sales reached a positive peak only when the demand reached a peak. And the demand for XYZ reached a peak only when maximum customers replaced the product. If a product like XYZ cannot survive the period of low sales, it may vanish from the market. Is the growth of a product in a mature market possible? Can it reach more number of customers despite the competition and maturity? Can a company that manufactures a mature product increase its revenue drastically? Through his article How Organizations Overachieve and Outrun Mature Markets (2005, p.1) in Ivey business journal, Al Magrath (director of corporate marketing, 3M Canada) says, it is possible. He illustrates the following ways of doing this task with examples: 1. Participate in a different value stream 2. Use technology to invent new sectors 3. Conquer new geographies 4. Extend branding power 5. Elongate your price/value 6. Help value changes in your market and 7. Use high end technology. Some companies also use cost reduction along with the above measures. Cost reduction, extending branding power, using high end technology to upgrade a product or a manufacturing process, acquisitio ns, mergers and asset reorganization form a part of what is called as corporate restructuring. As western automobile markets reached saturation, automobile giants like Chrysler and Volkswagen resorted to restructuring. Volkswagen had concentrated on its portfolio restructuring since early 90s. Volkswagen acquired Skoda in 1991. Volkswagen helped Skoda to emerge out of bankruptcy and Skoda soon became U.K.s best loved car (kn.theit.org 2009). This in turn helped Volkswagen, whose profits were declining around the same time. It gained access to the little penetrated car market of Eastern Europe. In 2009, it acquired 49.9% stake in Porsche. During recession, Porsche plunged into debts. Volkswagen used this opportunity to gain from its rival, who had a respected brand name globally (english.peopledaily.com.cn 2009). And now, Volkswagen is coming up with a strategy to acquire Toyota (in.reuters.com 2010). Even though the car market has matured in western parts of the globe, Volkswagen has been using strategic acquisitions to grow further. The financial restructuring process of Volkswagen, called as ForMotion is well-known. This restructuring process began in 2004. With the commencement of ForMotion, a number of workers lost their jobs. The working hours of most of the workers were extended (www.wsws.org 2006). Downsizing for restructuring had become infamous by then. A number of companies operating in mature market had adopted downsizing. Did the downsizing of its plants work for Volkswagen? Restructuring hurts Volkswagen was the headline of Los Angeles Times on October 28, 2006 (articles.latimes.com 2006). The profit of Volkswagen had plunged by 92% in the third quarter of 2005. Spokeswoman Christiane Ritz said the costs of employee buyouts and a deal with metalworkers union IG Metall to provide one-time payments of 6,279 Euros per person into workers pension funds were booked in the third quarter. But as demand for Volkswagen cars grew, the profit started rising. During the release of financial statements for the year 2005, the group chairman, Dr. Bernd Pischetsrieder, revealed the following facts: Profit before tax rose by 58.2 percent to à ¢Ã¢â‚¬Å¡Ã‚ ¬1.7 billion in 2005. Overall, however, the level of earnings we achieved remains unsatisfactory. Hence, the group decided to continue with the restructuring plan (volkswagenag.com 2006). But the true test of any company in the automotive industry was the global recession in 2008. Initially, in 2008, Volkswagen, too, felt the heat of the recession. But 2009 saw Volkswagen emerge as the only survivor in the automotive industry. Portfolio restructuring had helped Volkswagen to work in newer car markets. Even after the financial restructuring, its position was weak in the United States of America. But it proved to be a boon for Volkswagen. VW is coasting through 2009, boosting its share of global car sales to 12% from 9.9% in the first half of the year, reported time.com (2009). If Volkswagen had used only financial restructuring, it may have suffered major losses. It had invested money in financial restructuring, which was yet to be recovered through its profit. During recession, some of the smaller Skoda cars had performed better than the parent brand in the car markets (independent.co.uk 2007). But the cost reduction achieved through the financial restructuring comple mented the portfolio restructuring and Volkswagen survived. Thus, a mixture of restructuring strategies helped Volkswagen in alleviation of harm caused by the recession. The restructuring process surely helped Volkswagen in a mature market. But the sales have started stabilizing again. And this time, Volkswagen has come up with a new strategy. It has begun exploring hitherto unexplored markets. Initially, it was the cheap labour in India that attracted Volkswagen. But now, Volkswagen has set its eyes on the unexplored Indian car markets. Volkswagen began the execution of its plan with the launch of Polo in India. The Indian car market is predicted to be favourable for Volkswagen and is expected to generate additional revenues (thelocal.de 2009). Thus, it is true that restructuring (both financial and portfolio restructuring) has helped Volkswagen to not only survive in a mature market but also emerge as a leader in the automotive industry. But as observed from the study of Volkswagen, corporate restructuring can aid a mature industry only to a certain extent. It may help a company to perform better than its competitors in a mature market. It may also assist the growth of a company through acquisitions. But after a certain point, the company has to look for newer avenues of business. It is evident from the scenario in the automotive industry that restructuring of industries in a mature and cyclical product market is essential. Use of the right kind of restructuring, as in case of Volkswagen, is also important. But to supplement the growth of the product, exploration of newer markets is inevitable.

John Steinbecks East of Eden - Catherine as Monster :: East Eden Essays

Catherine as Monster in East of Eden In the novel, East of Eden by John Steinbeck, Catherine Ames is one of the main characters. She is introduced to the reader as a monster and as time goes on, she possesses both monster like and animal qualities. As Catherine she gets older and wiser, she gets more evil and displays her monster and animal like characteristics. She knows she is powerful and indestructible. She has manipulated and tricked many people her life causing them to go to the extreme... death. Catherine "Cathy" shows her evilness and her monster like behavior in many scenes throughout the book. Steinbeck illustrates Cathy as being a monster: "I believe there are monsters born in the world... It is my belief that Cathy Ames was born with the tendencies, or lack of them, which drove and forced her all of her life," (Steinbeck, 95-96). Cathy used this to her advantage by making people uneasy, but not so uneasy that they would not run away from her. Cathy was born with an innocent look that fooled many; she had golden blond hair, hazel eyes, a thin and delicate nose, and a small chin to make her face look heart shaped. According to the town Cathy lived, Cathy had a scent of sweetness, but that is just what Cathy wanted the town to see and think when Cathy planned her kill. "The fire broke out... the Ames house went up like a rocket†¦ Enough remained of Mr. and Mrs. Ames to make sure there were two bodies" (114-115). Cathy had set the house on fire and broke into the safe to steal the family's money. As the investigators scoped the place, they noticed that the bolts stuck out and there were no keys left in the locks. They knew it was not an accident. Cathy's body was never found, but the town assumed that she died. "If it had not been for Cathy's murder, the fire and robbery might have been a coincidence." Steinbeck, again, portrays the reader that Cathy is a monster: "When I said Cathy was a monster it seemed to me that it was so"(242). Steinbeck is reassuring the reader that Cathy is a monster and with the evidence before and after this statement. For example, Cathy later changes her name to Kate and runs a whorehouse.

Existentialism :: essays research papers

Existentialism Like "rationalism" and "empiricism," "existentialism" is a term that belongs to intellectual history. Its definition is thus to some extent one of historical convenience. The term was explicitly adopted as a self-description by Jean-Paul Sartre, and through the wide dissemination of the postwar literary and philosophical output of Sartre and his associates — notably Simone de Beauvoir, Maurice Merleau-Ponty, and Albert Camus — existentialism became identified with a cultural movement that flourished in Europe in the 1940s and 1950s. Among the major philosophers identified as existentialists (many of whom — for instance Camus and Heidegger — repudiated the label) were Karl Jaspers, Martin Heidegger, and Martin Buber in Germany, Jean Wahl and Gabriel Marcel in France, the Spaniards Josà © Ortega y Gasset and Miguel de Unamuno, and the Russians Nicholai Berdyaev and Lev Shestov. The nineteenth century philosophers, Soren Kierkeg aard and Friedrich Nietzsche, came to be seen as precursors of the movement. Existentialism was as much a literary phenomenon as a philosophical one. Sartre's own ideas were and are better known through his fictional works (such as Nausea and No Exit) than through his more purely philosophical ones (such as Being and Nothingness and Critique of Dialectical Reason), and the postwar years found a very diverse coterie of writers and artists linked under the term: retrospectively, Dostoevsky, Ibsen, and Kafka were conscripted; in Paris there were Jean Genet, Andrà © Gide, Andrà © Malraux, and the expatriate Samuel Beckett; the Norwegian Knut Hamsen and the Romanian Eugene Ionesco belong to the club; artists such as Alberto Giacommeti and even Abstract Expressionists such as Jackson Pollock, Arshile Gorky, and Willem de Kooning, and filmmakers such as Jean-Luc Godard and Ingmar Bergman were understood in existential terms. By the mid 1970s the cultural image of existentialism had becom e a clichà ©, parodized in countless books and films by Woody Allen. It is sometimes suggested, therefore, that existentialism just is this bygone cultural movement rather than an identifiable philosophical position; or, alternatively, that the term should be restricted to Sartre's philosophy alone. But while a philosophical definition of existentialism may not entirely ignore the cultural fate of the term, and while Sartre's thought must loom large in any account of existentialism, the concept does pick out a distinctive cluster of philosophical problems and helpfully identifies a relatively distinct current of twentieth- and now twenty-first century philosophical inquiry, one that has had significant impact on fields such as theology (through Rudolf Bultmann, Paul Tillich, Karl Barth, and others) and psychology (from Ludwig Binswanger and Medard Boss to Otto Rank, R.

Why Does Being A Philosopher Help In Philosophical Practice? :: Philosophers Philosophy Philosophical Papers

Why Does Being A Philosopher Help In Philosophical Practice? I think of personal consulting and much of what others call therapy as like dancing with another person. Sometimes you lead, sometimes you follow and sometimes the hardest part to learn is how to stay out of your partners way. You must understand your partners way of moving very well indeed if you are to follow it, move with it and know what will embellish and accentuate and what will interrupt. The 'dance' of personal consulting requires the kind of intellectual ability we should expect a well trained philosopher to have even if it also calls for traits and skills philosophers are not likely to have. Philosophers are specialists in understanding people who are notoriously hard to understand. What philosophers do is listen to and think about other philosophers and their job is to pit their minds against the minds of some of the greatest intellects our civilisation has known, 'understand which is not the same as memorising', what those thinkers did say, would say, avoided saying, assumed or presupposed; who influenced them and how; which ideas connect with which; and how it all fits together. They must be able to do this so well that they can take the part of any major philosopher, know that philosopher so well that they can become him or her 'from the inside'. All academic disciplines breed a degree of self criticism that goes with a sense of never knowing as much as one ought to know, but this is especially true of philosophy which has historically been one of the most self critical and introspective of disciplines. Indeed the popular image of not being good for much owes a great deal to philosophers criticisms of themselves from Socrates on, and perhaps the world at large would not have had enough imagination to change the discipline with being useless if the philosophers had not themselves intimated as much. It is no wonder that philosophers are insecure given the stature of the minds against which they must match wits and given the complexity of the problems with which they deal. Granted philosophers tend to be 'out of touch' with their feelings — a drawback in a consultant— which would be shared by representatives of other disciplines such as psychiatry, psychology or medicine, but they are well used to keeping themselves in focus, to bringing a question back to 'what do 1 make out of all this?

Properties of Enzymes and Competitive Inhibitors

Index Page Abstract†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦. †¦. †¦Ã¢â‚¬ ¦. 3 Introduction†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦. †¦. †¦Ã¢â‚¬ ¦.. 3 Materials and Chemicals used†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. †¦Ã¢â‚¬ ¦. †¦.. 3 Procedures†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. †¦Ã¢â‚¬ ¦ †¦Ã¢â‚¬ ¦ †¦Ã¢â‚¬ ¦ 4 Tables†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â ‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ 5-7 Results†¦Ã¢â‚¬ ¦.. †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ †¦Ã¢â‚¬ ¦8 Discussion†¦. †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ †¦Ã¢â‚¬ ¦8 Conclusion†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦. †¦Ã¢â‚¬ ¦. 8 Works Cited †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. 9 Properties of Enzymes and Competitive Inhibitors. Abstract: Properties of enzymes were found in this experiment and some other factors, which affect enzyme activity.Enzymes are catalyst; they catalyze very specific reactions. Results relating to the active site of specific enzymes played a big role while performing this experiment. The purpose of this experiment was to fin how inhibitors affect enzyme’s activity by competing for the active site against substrates. Introduction: Cells have the ability to perform chemical reactions that at normal temperature outside the body proceed too slowly to support life. Cells are able to perform some reactions rapidly because they possess protein catalyst called enzymes. Enzymes are proteins that catalyze (i. . , increase the rates of) chemical reactions. Each enzyme has a unique globular shape, a small portion of which functions as an active site capable of binding to specific reactants or substrates. It was hypothesized that enzyme concentration, temperature, and inhibitors will affect the properties and abilities of the enzyme. Materials: 1Wax Marking Pens 150 ml Beakers 3 400 ml Beaker 1 container of parafilm 1 set of 20 spec tubes 1 regular test tube rack 1 small test tube rack 1 box Kimwipes Eye Droppers 1 thermometer 2-10ml Graduated Cylinders 1 Spectrophotometer 7  °C waterbath with test tube racks Solutions: 1 flasks of pH 7 buffered ONPG 1 flask of Lactose 8% 1 flask of pH 7 buffered 1 flasks of 8% beta galactosidase Procedure 1. Obtain five test tubes and label them (i. e. A, B, C, D, E) 2. Using a 10 ml graduated cylinder put: Note: It is very important to add enzyme last. 1 ml of pH 7 buffered ONPG + No Lactose 8%(0ml) +(1 ml pH buffer) + Enzyme (1ml) solutions into tube A. 0% Lactose. 3. Using a 10 ml graduated cylinder put: 1 ml of pH 7 buffered ONPG + Lactose 8% (. 25ml) +(. 75ml pH buffer) + Enzyme (1ml) so lutions into tube B. % Lactose. 4. Using a 10 ml graduated cylinder put: 1 ml of pH 7 buffered ONPG + Lactose 8% (. 5ml) +(. 5ml pH buffer) + Enzyme (1ml) solutions into tube C. 4% Lactose. 5. Using a 10 ml graduated cylinder put: 1 ml of pH 7 buffered ONPG + Lactose 8% (. 75ml) +(. 25ml pH buffer) + Enzyme (1ml) solutions into tube D. 6% Lactose. 6. Using a 10 ml graduated cylinder put: 1 ml of pH 7 buffered ONPG + Lactose 8% (1ml) +(0ml pH buffer) + Enzyme (1ml) solutions into tube E. 8% Lactose. 7. Cover each of the tubes with parafilm and place the tubes in the 37  °C waterbath for 30 minutes. . After 30 minutes, determine if the reaction has occurred in each tube, and notice change in color. 9. Test tube E acted as our control test tube because no competitive inhibitor was added. Lactose was the competitive inhibitor for this reaction into the test tube. Note: Because the result on steps 4 and 6 were not accurate for our particular experiment, steps 4 and 6 were performed twi ce. The following table and graph express the results after the measurements and mixing. Table 1. Measurements after mixing the solutions into the test tubes.Solutions| pH 7 Buffered ONPG (ml)| Lactose 8% (ml)| pH buffer (ml)| Enzyme B-Gal (ml)| Total amount of mls. | Test tube A| 1| 0| 1| 1| 3| Test tube B| 1| 0. 25| 0. 75| 1| 3| Test tube C| 1| 0. 5| 0. 5| 1| 3| Test tube D| 1| 0. 75| 0. 25| 1| 3| Test tube E| 1| 1| 0| 1| 3| This table represents the total amounts of each solution added to each test tube in order to get 3 mls for each test tube. This table is used only to represent how the result will look like. Graph 1. Measurements after mixing the solutions into the test tubes. This graph depicts the contents inside the test tubes after mixing the mentioned solutions.Measurement of O-nitrophenol. (ONPG) Although the appearance of yellow in the tubes indicated that O-nitrophenol was present, the color, alone, did not tell us how much was present. It was possible to measure the a mount of O-nitrophenol present by measuring the intensity of the yellow with a spectrophotometer. 1. The contents of the 5 tubes were poured into spec 20 tubes. The positions were labeled, but the spec tubes were left clear in order to have an accurate measurement absorbance. 2. Test tube E acted as the control tube for this, since that tube did not contain inhibitor.Note: Absorbance 420nm in this experiment will be a measure of the concentration of the O-nitrophenol molecules in each of the solutions. Using the Spectrophotometer The spectrophotometer was an instrument designed to measure the amount of light transmitted through solutions, or absorbed by substances in the solution. Light of a specific wavelength is emitted from a special bulb and passed through a tube containing a substance solution. The greater concentration of those particles; the greater the absorbance. It is very important to select the most appropriate wavelength of light for use.These procedures were followed i n order to set up the Spectrophotometer. 1. 420 nm was the wavelength to use in the inhibitor experiment lab designed because O-nitrophenol maximally absorbs a light at 420. 2. The Spectrophotometer was zeroed out with the control knob so that the needle reads 0% transmittance on the upper scale. 3. The control tube A was put in the holder, and the lid was closed. The light control knob was adjusted so that the needle could read 100% transmittance. 4. The control tube was removed from the holder. The lid was then closed noticing the needle again read 0% transmittance. 5.All other test tubes were placed into the Spectrophotometer and read as well. 6. Data for these results was recorded on the following table. Table 2. Effect of competitive inhibitor concentration lactose on the production of O-nitrophenol. Effect of Competitive Inhibitor Concentration on production of ONGP Product| Tube| Inhibitor Concentration| Intensity of yellow| Absorbance| ? moles of ONPG produced/30min | ? mole s of ONPG produced/min| A| 0%| ++++| 1. 55| 38. 75| 1. 291666667| B| 2%| +++| 0. 43| 107. 5| 3. 583333333| C| 4%| ++| 0. 13| 32. 5| 1. 083333333| D| 6%| +| 0. 02| 5| 0. 166666667| E| 8%| 0 | 0| 0| 0|Calculation of ? moles O-nitrophenol produced per minutes. Ex. Tube A: ? moles of ONPG produced/30min Absorbance/0. 004= ? moles of ONPG produced per 30min 0. 155 / 0. 004= 38. 75 ? moles Ex 2 Tube A: ? moles of ONPG produced/min ?moles of ONPG produced per 30min/ 30min 38. 75 /30=1. 291666667 ? moles of ONPG produced/min From the absorbance data that was measured the O-nitrophenol produced per minute was calculated. 1. Each ? mole of O-nitrophenol produced an absorbance of 0. 004. The absorbance measured was divided by 0. 004 to determine the number of ? moles produced during the experiment.The values were recorded in table 2, fifth column. 2. The measurements that were obtained in the fifth column were divided by 30(number of minutes left in the waterbath) to obtain the number of ? mol es of O-nitrophenol produced per minute. Graph 2. Absorbance measurements for inhibitor concentration lactose on the production of O-nitrophenol. Absorbance Absorbance Test Tubes Test Tubes Results According to the hypothesis that temperature, enzyme concentration, and concentration will affect the properties and functions of the enzymes. The hypothesis was supported because graph and tables express the change in absorbance, and ? oles produced. Discussion The tables were able to depict the result in order to get better and accurate results for this particular experiment. Measurements have to be performed with precaution, making sure the enzyme and the contents are mixed properly and at the same time. Conclusion Enzyme activity can be affected by other molecules. Inhibitors are molecules that decrease enzyme activity; activators are molecules that increase activity. Activity is also affected by temperature, chemical environment, change in pH, and the concentration of substrate.